I am currently looking into doing double-labelling of pig brain tissue
for neuropeptides using two rabbit antibodies and a two fluorescence
detection system. I am considering using unlabelled fab fragments to
hide the first primary.
If anyone has experience (either good or bad) with this technique, I
would appreciate references and/or advice that could make this job a
little easier. Thanks.
Please send responses to: rambis at cvm01.vm.iastate.edu
Paul L. "Pro" Pearson
President, Kurt Rambis Fan Club (unofficial)
Ames, IA Chapter