TRANSFECTION OF CULTURED NEURONS

[Matthew Frosch]

Does anyone have experience with transient transfection of neurons
in primary culture?  I am interested in introducing a series of
b-gal reporter constructs into cortical neurons in established cultures
(i.e., from day E15-17 gestation rats, maintained for 3-5 weeks).
Specific questions:
         What methods seem to work?
         What is a good normalization control?
         What control promoters work well?

Any advice will be appreciated.

Thanks,

Matthew Frosch
frosch at dsg.harvard.edu