We are immunophenotyping by flow cytometry normal mouse brain cells
and find that while we can easily detect certain
phenotypes in cell suspensions (either homogenized or
following trypsin digestion), in situ detection (frozen
or fixed) is very difficult. We are new to the brain
and wonder whether others doing similar analyses have
had problems of this nature and if so, is there a way
around the problem.
Any advice would be greatly appreciated - thanks.
William S. Walker, Ph.D.
Department of Immunology
St. Jude Children's Research Hospital
Memphis, Tennessee USA 38105-2794
bill.walker at stjude.org
Voice: (901) 495-2537
FAX: (901) 495-3107