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FBS trouble

jcoleman at msvax.mssm.edu jcoleman at msvax.mssm.edu
Sun Nov 5 00:12:20 EST 1995

In article <47e2q2$g4g at larch.cc.swarthmore.edu>, ivette emery <iemery1 at cc.swarthmore.edu> writes:
>I have had contamination in the last three bottles of FBS that I 
>purchased from GIBCO (Life technologies).  After plating out 3 mls 
>of FBS from previously unopened bottles, and incubating at 25oC 
>overnight I see tiny organisms; I think is bacteria.  These little 
>bugs go through 0.2um filters and are resistant to the Penn/Strep 
>in my media.  I have been extra careful in handeling the FBS in my hood
>to be sure I am not introducing the contamination myself and I have 
>scrubbed my incubator to make sure it is not coming from there.  
>Luckily, the replacement bottle that GIBCO sent me, that came in a 
>plastic bottle, as opposed to the glass bottles I had, seems free of
>my mysterious bug.  Has anyone else had this problem?
Nope...never had contamination problems with GIBCO stuff...but we use Hyclone
serum...never had any trouble with the Hyclone brand. Most bugs we have are
resistant to Pen/Strep I think...because everyone uses it :-)

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