FBS trouble

jcoleman at msvax.mssm.edu jcoleman at msvax.mssm.edu
Sun Nov 5 00:12:20 EST 1995


In article <47e2q2$g4g at larch.cc.swarthmore.edu>, ivette emery <iemery1 at cc.swarthmore.edu> writes:
>I have had contamination in the last three bottles of FBS that I 
>purchased from GIBCO (Life technologies).  After plating out 3 mls 
>of FBS from previously unopened bottles, and incubating at 25oC 
>overnight I see tiny organisms; I think is bacteria.  These little 
>bugs go through 0.2um filters and are resistant to the Penn/Strep 
>in my media.  I have been extra careful in handeling the FBS in my hood
>to be sure I am not introducing the contamination myself and I have 
>scrubbed my incubator to make sure it is not coming from there.  
>Luckily, the replacement bottle that GIBCO sent me, that came in a 
>plastic bottle, as opposed to the glass bottles I had, seems free of
>my mysterious bug.  Has anyone else had this problem?
>
>Ivette
>
Nope...never had contamination problems with GIBCO stuff...but we use Hyclone
serum...never had any trouble with the Hyclone brand. Most bugs we have are
resistant to Pen/Strep I think...because everyone uses it :-)
Jen



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