SAPORIN !?

[User Name]

Is there anyone out there who can help me?

I am a PhD student at The Institute of Psychiatry working on a selective mechanism 
for removing cholinergic neurons from the basal forebrain in an attempt to model 
Alzheimers Disease. I am using a new immunotoxin called Saporin. Thus far I have 
carried out 3 pilot studies - to assess which dose removes the neurons but leaves no 
other damage. Unfortunately Chemicon (the company which distributes the toxin) seem 
to be having problems and keep changing both the concentration of the stock 
solution, but more importantly the activity.

Is there anyone who is currently using this toxin successfully?

The dose that the company recommend I use are not accurate and even at 3 times this 
dose there appears to be no effect.

Am I flogging a dead horse or can anyone help me!

Any comments or advise especially from anyone using this stuff would be much 
appreciated.

Many thanks

TRACYANN (E-mail : spjutap at iop.bpmf.ac.uk)