Protocol for Western and Northern blotting

Abdelhamid Benazzouz Abdelhamid.Benazzouz at ujf-grenoble.fr
Wed Oct 4 07:43:20 EST 1995


I'm would like to detect bcl-2, bax, p53, Fas and Ras protein and RNA 
by western and northern blotting

Antibodies I use for bcl-2 are polyclonal, p53 monoclonal (Boehringer),
bax monoclonal (Tebu) and Fas from transduction laboratories. I need a protocol
for each one.
I need also sequence for probe for each one for Northern Detection and 
primers sequences for RT-PCR detection

The cells used are PC12

Thank you very much

David BLUM



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