HELP! Thyrosine Hydroxylase HELP!

Michael Clark michael-clark at uiowa.edu
Tue Sep 12 00:34:59 EST 1995


Andrew P.T.Rhyn wrote:
>....Are there any cells suitable as positive controls for Thyrosine 
>Hydroxylase?....

Try using PC12 cells (an adrenal chromaffin derived cell line) that
have been treated with NGF (25-50ng/mL media).  These are known to
express TH and have been used in many studies of that enzyme.  They are
available from many sources, probably even a nearby lab.  BTW: PC12's
are from rats, so your antibody will need to recognize rat TH.

Michael Clark
University of Iowa



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