Immunofluorescence staining of cell membranes ?

Michael P. Maher maher at cco.caltech.edu
Fri Sep 15 12:14:01 EST 1995


In article <439fid$3uh at selene.rz.uni-duesseldorf.de>,
goran at atax.neur.uni-duesseldorf.de (G.P.) wrote:

> What is commonly used to stain immunohistochemically cell membranes ?
> 
> I'd prefer not to have staining of nuclear and other intracellular
> membranes, but could live with it if absolutely necessary.
> 
> Goran


Can't you just use a membrane-bound dye, like DiI?  It's easy to use and
bright, and it only stains the outer cell membrane until it gets
internalized after a few days.



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