Immunofluorescence staining of cell membranes ?
spkaslm at ucl.ac.uk
Fri Sep 15 15:24:47 EST 1995
Hello again Goran.
I apologise for the delay; I tried to contact you on e-mail but our
server did not recognise your address. After a brief conversation with my
boss who is an expert in such matters, the best way of visualising the
membrane under the light microscope is to stain membrane ATP-ase.
Apparently the membrane can also be stained with Ruthenium Red which is
visible under the light microscope and appears electron dense under the
electron microscope. Both techniques are applicable to histological
specimens and the boss tells me that he even has some protocols somewhere.
If I can catch him at a convenient moment I can ask him for them and fax
them to you if you are interested.
Hope this is of some help
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