In article <4km8lt$hbo at copland.udel.edu>,
Thomas R. Gregg <greggt at copland.udel.edu> wrote:
>Hi, I am helping teach an undergraduate course in neuroscience.
>Does anyone know of a good, up-to-date 10-20 page handout on histological
>techniques, including staining? This handout should cover c-fos,
>autoradiography, and IHC as well as older techniques.
tom,
while maybe not exactly what you are looking for,
there is a chapter (#5, Methods of Physio Psych) in
Neil Carlson's _Physiology of Behavior_ (5th ed, 1994) that
might be of interest.
the total chapter is 32 pages; topics include:
Neuroanatomical and Neurochemical Techniques
Histo
Tracing connections
Localization of Receptors/Neurochemicals
Measuring metabolic activity
Microdialysis
CT/MRI/PET
Electron microscopy
Experimental Ablation
Lesions
Stereotax
Electrophysiology
recording
stimulation
the neurochem part has some really nice color photos of IHC and I have
found it to be a good general resource for basic/general methodology
of neuroscience.
usual disclaimers apply.
hope this helps
cheers,
Josh Rodefer
University of Minnesota
*****
rode0008 at gold.tc.umn.edu