In article <4kmgmm$a6r at credit.erin>,
James Fullard <jfullard at tuzo.erin.utoronto.ca> wrote:
>Does anyone have any experience using Lucifer yellow on fixed
>insect nervous tissue? There have been some studies using
>vertebrates and molluscs but I've never seen anything with insects. I'd
>like to backfill some very small axons in a motor branch of a moth and
>using fixed tissue would make isolating the nervelets a lot easier.
>>Thanks in advance.
>
Have you thought of using DiI or similar product from Molecular Probes
(e.g. DiO)? They're supposed to work very well in fixed tissue.
Brian
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Brian Scott | "In other studies you go as far as others have gone
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