Staining neurons in fixed tissue
brians at interlog.com
Sun Apr 14 13:02:11 EST 1996
In article <4kmgmm$a6r at credit.erin>,
James Fullard <jfullard at tuzo.erin.utoronto.ca> wrote:
>Does anyone have any experience using Lucifer yellow on fixed
>insect nervous tissue? There have been some studies using
>vertebrates and molluscs but I've never seen anything with insects. I'd
>like to backfill some very small axons in a motor branch of a moth and
>using fixed tissue would make isolating the nervelets a lot easier.
>Thanks in advance.
Have you thought of using DiI or similar product from Molecular Probes
(e.g. DiO)? They're supposed to work very well in fixed tissue.
Brian Scott | "In other studies you go as far as others have gone
brians at interlog.com | before you, and there is nothing more to know; but
M.Sc. student in | in scientific pursuit there is continual food for
Neurophysiology | discovery and wonder." - Victor Frankenstein
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