help with cryostat cutting

jcoleman at msvax.mssm.edu jcoleman at msvax.mssm.edu
Thu Apr 18 09:47:56 EST 1996


In article <4l375j$qt4 at amgen.amgen.com>, none <none> writes:
>I'm no expert, in fact, I'm not even skilled in this myself.  But
>just based on what you describe, it sounds like a problem with
>the smoothness of the blade-falling-action, if you know what I
>mean.  Is this a manual one, or automatic?  I've only even tried
>cutting a few times myself, and found it very frustrating, and the
>problem I encountered most was the one you describe.  I always
>ascribed it to the speed/motion of the blade across the sample.
>Perhaps this is just too simplistic, especially since you have
>years of experience.
>
>
>*** Disclaimer: These are the opinions of the poster not Amgen Inc.***
Likewise, I am no expert in fact I am most likely to question my choice of
science as a career when cryostating ...could it be poor sucrose infiltration?
or perhaps the block itself is too cold.

Just a feeble thought :-)
Jen



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