Receptor Binding

[Han NianLin]

Dear Colleagues,

I am doing some research about angiotensin II receptor in brain. To 
terminate the reaction between the radioligand and the membrane, there 
are two major methods: centrifugation, filtration. People usually use the 
filtration because it is easier and faster. My question is whether the 
filtration will lose some very high affinity binding sites because the 
background is very high? Is centrifugation able to detect the extremely 
high affinity binding sites?

Thank you in advance for your kind replies. I am looking forward to 
receiving your precious suggestions.

Your sincerely,

Han