Organotypic cultures and Fura-2AM

Chris hatton at
Thu Aug 22 19:21:32 EST 1996

I have a question needing intellectual thought and practical experience,

I'm working with organotypic cultures, and specifically using them with
Fura-2.  We have problem with membranes absorbing at 340nm, any
suggestions how to get round this would be most appreciated.  Any way of
not using membranes would be even better, the cultures are brain, but
other parts of body also welcome.  Please reply either by Email or, to
discussion group.


Hatton at

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