Neuronal tracing with DiI
liamgray at interalpha.co.UK
Thu Dec 5 19:47:49 EST 1996
Jurgen Hens <jhens at ruca.ua.ac.be> wrote:
>One of the subjects of my research is to trace neurons with the
>fluorescent dye DiI. I would like to do this by precipitation of DiI
>onto glass beads (75µm), which subsequently are applied onto the neurons
>of interest. My question is the following: does anyone have some
>suggestions how I can precipitate different layers of DiI crystals onto
>one glass bead?
Try directly applying a crystal with a tungsten micro electrode. Its
much finer than applying it with a glass bead. If you want to apply
different Di crytal types then simply repeat for each crystal type.
If this is no good I have read an article where the authors applied Di
crystals using a glass microelectrode encrusted with crystals. They
simply broke the laden tip when in the correct position and left it
there. Doesn't sound very "atraumatic" but there it is!
liamgray at interalpha.co.uk
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