luciferase assay for ATP

Jim Ryan ryan at hws.edu
Wed Feb 7 13:19:03 EST 1996


Is any one familiar with the use of luciferase for the detection of
intracellular ATP in cells?  I need to try to determine if ATP levels are
increasing or decreasing in single cultured neurons.  If anyone has a
protocol or can help me with this I'd greatly appreciate it.  Jim

____________________________________________________
Dr. James M. Ryan                       Ph: 315-781-3601
Biology Department                      FAX: 315-781-3587
Hobart and William Smith Colleges
Geneva, NY  14456                       ryan at hws.edu





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