Patch Clamping Smooth Muscle?

[Scott Hancock]

I'm trying to do a whole cell patch clamp study on smooth muscle cells 
isolated from rat middle cerebral artery. However the cells when 
isolated, have a very inelastic, noncompliant membrane, and I have been 
having trouble getting good seals. Can anybody suggest a reason for this 
problem (solution osmolarity ?) ?

 Scott Hancock 4330 
faculty of medicine Health Sciences Center Memorial University of NFLD
A1B-3V6  
"knowledge is the spice of life but ignorance is bliss."