Thionin staining

Igor Polyakov Igor_Polyakov at QMGATE.ARC.NASA.GOV
Wed Feb 28 21:53:18 EST 1996


Mail*Link(r) SMTP               Thionin staining

 Robert L. Hoskins wrote:
> I've stained some brain sections with thionin, and, after coverslipping
>the slides, the stain appears to have faded considerably.  Anyone have a
>clue as to why this might happen?  Can I remove the coverslips with xylene
>and re-stain the sections?

>Thanks,
>blh

Can you send me an information what kind of the sections have you tried to
stain (e.g. frozen, fixed, unfixed, paraffin ...)?
Igor Polyakov
Igor_Polyakov at qmgate.arc.nasa.gov





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