JarOfFlies at gnn.com wrote: : Hi there, : : can anyone tell (or refer) me how to isolate CA1 neurons from neonatal rat? : : Thanks, : Fred : For a good start, try _Culturing Nerve Cells_, Banker G and Goslin K, eds. -- Jim Hutchins hutchins at netdoor.com hutchins at umsmed.edu http://fiona.umsmed.edu/~hutchins/