A question about in situ hybridization

Martin Downes martind at magi.com
Wed Mar 13 12:52:36 EST 1996


I need some help.

I am trying to do in situ hybridization on parrafin embedded
sections with a viral RNA target. I have used cRNA probes 300
900 + 1400 bas and oligo probes. These probes work fine in
other systems but connot get a positive signal in the above
system.

Has anyone else experienced this problem and what did you do?
Any suggestions would be welcome. Please respond by email.

Thanks,

Mary Downes
Ottawa, Canada



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