Plastic embedded Immunohistochemsty

Gregory S. McGillem mcgillem at vision.vsrc.uab.edu
Thu Mar 28 16:21:42 EST 1996


I am trying to immunostain retinal tissue that has been embedded in a plastic 
and then sectioned at 3-6um.  I have tried both JB-4 and Immunobed versions of 
glycol methacrylate and I have not gotten any immunoreactivity.  I know the 
antigen sites are present prior to embedding.  

Does anyone have any helpful hints on staining plastic embedded tissue?

Greg Mc.

mcgillem at vision.vsrc.uab.edu



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