Best Method of Brain Dissection
jerikse at bsd.meddean.luc.edu
Sun Nov 10 12:33:49 EST 1996
pillers at med.unr.edu wrote:
>I am attempting neocortex studies in rat and am concerned that I may not be
>doing the best job I can in dissecting the brain. Could anyone give me a good
>procedure (I am using halothane to anesthetize then decapitation) or recommend
>a resource I could use? Any help would be appreciated.
Although you haven't stated what exactly you are doing (are you
looking for a way to anesthetize the rat, wondering how to remove the
cortical mantle or doing cryostat cuts; what is the age of the rat,
etc.), the following advice from someone who has done a lot of
cortical dissections will help:
INSTRUCTIONS FOR PERFECT DISSECTIONS
0. Find someone who does dissections in your department and ask them
to show you the method that they use.
2. Repeat 1 as necessary.
If, after faithfully following the instructions above for a week, your
dissections are still bad, seek the advice of a higher guru in your
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