DIG vs 35S for in situ hybridization
william at neuro.usc.edu
Mon Oct 7 16:12:04 EST 1996
I wish to do some in situ hybridization studies looking at the expression
of a specific gene in rat and rabbit brain sections. I would like to
know the advantages and disadvantages of using DIG and 35-S as the means
for detection. Also, I am considering using riboprobes. If you have
reasons as to why DNA oligos might work better, please let me know.
Thanks for any advice/suggestions.
William Sun, Ph.D. Phone: (213)740-3406
Neuroscience Program FAX: (213)740-5687
University of Southern California Pager: (310)243-9878
Los Angeles, CA 90089-2520 http://www-bcf.usc.edu/~wisun/
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