DIG vs 35S for in situ hybridization

Gabriele Kerber kerber at alf.biochem.mpg.de
Wed Oct 16 05:14:27 EST 1996


Ms. K.K. Martin wrote:
> 
> I have used both 35S and DIG for ISHH.  I far prefer DIG and will never,
> ever use 35S again.  DIG is easier, safer, faster (results in hours rather
> than days or weeks you get good spatial localisation and, best
> of all, you avoid all that nasty paperwork associated with keeping track
> of your radioactive waste.  Boehringer Mannheim have very helpful manuals
> for using DIG in ISHH.  Good luck.


DIG is wonderful for IHS with riboprobes. But if you want to use 
oligonucleotides, you will probably have a lot of problems with 
DIG. With oligos it´s better to use 35S or even 32P.

Gabriele



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