Electrically injecting neurons

Vidya Chandrsekaran vc at acsu.buffalo.edu
Thu Sep 12 12:53:40 EST 1996


I'm working on a project to label neurons of a sympathetic ganglia by
electrical intracellular injections. The magnification I can work at on my
microscope is either 4X or 10X.The ganglia is pinned on sylgard in a 35mm
dish.AS I cannot see the cells, I have a difficulty getting cells. I've
tried using action potential to detect the but it is difficult to see
action potential. Any suggestions on how to make the technique work, would
be appreciated. 

Thanks 
vidya





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