"CNS specific knock-outs for cre-lox"
Thomas R. Anderson
babco at ix.netcom.com
Tue Sep 24 04:51:47 EST 1996
>In article <525sf1$scg at sun0.urz.uni-heidelberg.de>,
>un691cs at genius.embnet.dkfz-heidelberg.de (z) wrote:
>> here in the lab we would like to make CNS specific knock-outs
>> with the cre-lox system. For this, I am looking for a CNS (not
>> PNS !) specific promoter, which is either expressed only in CNS
>> neurons, or in CNS neurons as well as glia. neurofilament, 14-3-3,
>> vimentin, nestin or negrin won't do, as these are either
>> expressed in PNS neurons or outside by non-neural cells as well.
As an aside, for those of you using the cre-lox system, Berkeley
Antibody Company (BAbCO) has a monoclonal antibody against cre
recombinase. This antibody can be useful for confirming expression
patterns of the enzyme.
If you want more information, please send an email.
Berkeley Antibody Company (BAbCO)
tel: 1-800-922-2226 (in the USA)
email: tanders at babco.com
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