mouse monoclonal to detect mouse proteins
lgd450 at nwu.edu
Fri Apr 11 16:03:06 EST 1997
In article <Pine.BSF.3.95.970411150751.15161B-100000 at orion.webspan.net>,
"French A. Lewis" <falewis at mail.webspan.net> wrote:
> I have a question for those who have done immunohistochemistry on
> mouse brain slices. My question is how to detect the antibody that has
> bound. I have done western blots, and have used either a conjugated
> goat-anti mouse or a goat-anti rabbit as a secondary antibody to visualize
> where my primary antibody has bound to proteins on the nitrocellulose. I
> am wondering, if using a goat-anti mouse IgG would lead to high background
> in IHC due to the presence of endogenous mouse IgG in the brain.
> Thanks in advance for the help,
Using a mouse antibody on mouse tissue can definitely cause background.
For the best bet, use a polyclonal antibody made in a different animal,
such as a rabbit, goat, or chicken. You can still have problems with
cross reactivity if you antibody is made in rat.
Insanity runs in my family. It practically gallops.
--From "Arsenic and Old Lace"
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