GFP expression

Jean-Philippe Hugnot spjtjph at
Wed Feb 12 16:15:52 EST 1997


I bought from clontech a plasmid which express a new form of green
fluorescent protein showing a brighter signal (vector pEGFP). In fact
after transfection in Cos or in the cell lines we are working with
(neuroepithelial cells) we have had very bright cells. The problem came
up when we attempted to derive stable GFP expressing cell lines and till
now we didn't manage to generate such cell lines.

has anybody managed to do that and could give me some tips

Thank you 

Hugnot J.P.
Institute of Psychiatry
Department of psychology
De Crespigny Park
Denmark Hill
London SE5 8AF
fax: 0171 708 3497
tel: 0171 919 34 00 or 32 33
email:spjtjph at

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