We are trying to double stain sheep tissue using 2 rabbit polyclonal
antibodies. The antigens are fluorogold and CRH. The problem is that
they both are in the cytoplasm. Using DAB and Ni-DAB it is hard to
differentiate double from single stained cells. We tried fluorescence
but for some reason we got beautiful fibers but no double stained cells
as we expected. Any suggestons?