marker for layers

Joseph Strout jstrout at ucsd.edu
Wed Jan 15 11:28:25 EST 1997


On Wed, 15 Jan 1997, Ekkehard Lange wrote:

> I`m looking for markers to label single cortex layers
> during development in rodent brain.
> I hope somebody can help me wich antibody I have to use.

I don't know about single layers, but if you stain with propidium iodide
(PI), the result appears very similar to a Nissl stain.  Experienced
anatomists can perceive the layers in such stains.  PI has broad
absorption and emission spectra, so you have to stimulate it separate from
the other channels, but it works.

,------------------------------------------------------------------.
|    Joseph J. Strout           Department of Neuroscience, UCSD   |
|    jstrout at ucsd.edu           http://www-acs.ucsd.edu/~jstrout/  |
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