Cleaning Primary cultures

Andrea LeBlanc mdal at musica.mcgill.ca
Fri Jan 24 07:01:37 EST 1997


In article <5c5dli at news-ipg.umds.ac.uk>, Steve Bunting
<s.bunting at UMDS.ac.uk> wrote:

> Dear all,
> 
> I am currently trying to isolate cells from adult rat striatum, but I am 
> obtaining large amounts of debris (myelin etc). Does anyone know how I 
> can "clean up" my primary cultures.
> 
> Any help will be certainly be appreciated.
> 
> Steve

Hi Steve,

You can try some kind of centrifugation to remove the lipids. I think that
sucrose gradients, Ficoll, Nycodenz or optiprep may help you (these are
available from Pharma).

Good luck,

Andrea.

-- 
Andrea LeBlanc



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