Cleaning Primary cultures

Andrea LeBlanc mdal at
Fri Jan 24 07:01:37 EST 1997

In article <5c5dli at>, Steve Bunting
<s.bunting at> wrote:

> Dear all,
> I am currently trying to isolate cells from adult rat striatum, but I am 
> obtaining large amounts of debris (myelin etc). Does anyone know how I 
> can "clean up" my primary cultures.
> Any help will be certainly be appreciated.
> Steve

Hi Steve,

You can try some kind of centrifugation to remove the lipids. I think that
sucrose gradients, Ficoll, Nycodenz or optiprep may help you (these are
available from Pharma).

Good luck,


Andrea LeBlanc

More information about the Neur-sci mailing list