Gelatin embedding of mouse brain for vibratome sections
J. A. Kiernan
jkiernan at panther.uwo.ca
Fri Jul 18 15:25:32 EST 1997
In article <5qo90g$bfb at lace.colorado.edu> "Edoardo \"Dado\" Marcora" <marcora at colorado.edu> writes:
>I would be pleased if someone could let me know which concentration of
>gelatin to use in order to embed mouse brains (fixed). I will make vibratome
>sections out of them.
You probably don't need to embed in gelatin, but if you
want to, soak the fixed specimen in about 25 ml of 5%
gelatin in a 50 ml beaker at 37øC overnight. Put in
fridge (4øC) until set (2 hours), then cut out a block
and immerse it in any formaldehyde fixative, for 24 hours.
Finally, wash in running water for a few hours to get
rid of excess formaldehyde. The fixation after embedding
is to make the gelatin insoluble in water.
John A. Kiernan
Department of Anatomy
Univ. of Western Ontario
LONDON, Canada N6A 5C1
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