Hi I'd like to perform perforated patches with Gramicidin. Actually I tried out a receipe where G. is solved in DMSO (5 mg/1ml). when I add this stock to my pipette solution the G. falls out. Using Methanol instead of DMSO I had the same result. Now: Why? My pipette solution contains KCl, CaCl2, EGTA, MgCl2, Na2ATP and HEPES. Thanks in advance Udo Kraushaar