Perforated patches - how?

[Robert Alexander Reiprich]

Udo Kraushaar wrote:
> 
> Hi
> 
> I'd like to perform perforated patches with Gramicidin. Actually I tried out a
> receipe where G. is solved in DMSO (5 mg/1ml). when I add this stock to my
> pipette solution the G. falls out. Using Methanol instead of DMSO I had the
> same result. Now: Why?
> My pipette solution contains KCl, CaCl2, EGTA, MgCl2, Na2ATP and HEPES.

Did you try lower concentrations of Gramicidin or did you sonify your
solution after dissolving?
Alex