(no subject)

Colette beacol at douglas.mcgill.ca
Fri Jun 13 12:28:47 EST 1997


Udo Kraushaar wrote:
> 
> Hi
> 
> I'd like to perform perforated patches with Gramicidin. Actually I tried out a
> receipe where G. is solved in DMSO (5 mg/1ml). when I add this stock to my
> pipette solution the G. falls out. Using Methanol instead of DMSO I had the
> same result. Now: Why?
> My pipette solution contains KCl, CaCl2, EGTA, MgCl2, Na2ATP and HEPES.
> 
> Thanks in advance
> 
> Udo Kraushaar
Udo Kraushaar wrote:
> 
> Hi
> 
> I'd like to perform perforated patches with Gramicidin. Actually I tried out a
> receipe where G. is solved in DMSO (5 mg/1ml). when I add this stock to my
> pipette solution the G. falls out. Using Methanol instead of DMSO I had the
> same result. Now: Why?
> My pipette solution contains KCl, CaCl2, EGTA, MgCl2, Na2ATP and HEPES.
> 
> Thanks in advance
> 
> Udo Kraushaar



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