RT-PCR HEEELP!

Marta Radman-Livaja marta.radman-liavja at zg.tel.hr
Mon Mar 24 15:58:03 EST 1997


I am trying to amplify the 5HT-2A mRNA from total rat platelet RNA with the
RT-PCR method. I have just once obtained a faint band on EtBr agarose gel.
Even though I used the same protocol for other samples, my labor has been
in vain. 
Can anyone who succeeded more than once in a similar experiment contact me
and give me a few tips. I'd be very grateful 




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