dii and background fluorescence.

shan s_bentz at hamlet.uncg.edu
Mon Nov 10 11:55:39 EST 1997


Hello all.  

I'm using dii in starling brains and I'm noticing that with a rhodamine
filter (in order to visualize dii), fiber tracts/bundles appear to be much
brighter than their surroundings, even if they (apparently) have no dye in
them.  Is this normal?  Do fibers normally autofluoresce?  Any advice,
thoughts or opinions on this would be most gratefully received.  

All the best, 
scb


~~~~~~~~~~~~
S. C. Bentz
UNC-Greensboro
Psychology Department
P.O. Box 26164
Greensboro, NC  27402-6164
s_bentz at hamlet.uncg.edu or scbentz at goodall.uncg.edu
http://www.uncg.edu/~s_bentz/index.html
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