I am planning to begin some experiments concerning the use of 10
micron sections (coronal) to observe G-protein activation. I have two
methodological questions that I hope someone can answer:
Can I freeze/thaw my sections without having any significant effects
concerning G-protein function, if yes what would the time course be?
I will be putting excess GDP [(2mM) before actually putting the
slice in contact wit the radioactive ligand] for better binding of
my ligand should I try an external source of heat to (hair dryer?)
avoid an undesirable dilution effect after this phase.
Thank you in advance for your replies - Joe