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serotonin and HPLC

LeBron Russell alrussell at utc.campus.mci.net
Sun Oct 19 10:28:41 EST 1997

Laurent Naudon wrote:

> I want to measure 5HT in several brain structures. I use perchloric acid 0.1M to prepare the samples. In this
> condition a degradation of the 5HT seems to occur during the preparation of the samples. How may I improve the
> stability of 5HT?
> Laurent Naudon PhD

  Perhaps you can use a phosphate buffer or some other buffer to stabilize a neutral pH.

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