Hi, I want to do In Situ Hybridisation on mouse brain sections using DIG-labelled RNA probes. Problem is my brain tissue has been collected, frozen in liquid N2 and then stored at -80 without any pre-treatment. Can anyone give me advice on the best method to use for ISHs on brain tissue stored in this way? Thanks in advance. Gino Miele, gino.miele at bbsrc.ac.uk