Method for ISH

gino.miele gino.miele at
Sat Apr 11 05:44:31 EST 1998

Hi, I want to do In Situ Hybridisation on mouse brain sections using DIG-labelled 
RNA probes.  Problem is my brain tissue has been collected, frozen in liquid N2 and 
then stored at -80 without any pre-treatment.  Can anyone give me advice on the best 
method to use for ISHs on brain tissue stored in this way?  Thanks in advance.

Gino Miele,

gino.miele at

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