Method for ISH

gino.miele gino.miele at bbsrc.ac.uk
Sat Apr 11 05:44:31 EST 1998


Hi, I want to do In Situ Hybridisation on mouse brain sections using DIG-labelled 
RNA probes.  Problem is my brain tissue has been collected, frozen in liquid N2 and 
then stored at -80 without any pre-treatment.  Can anyone give me advice on the best 
method to use for ISHs on brain tissue stored in this way?  Thanks in advance.

Gino Miele,



gino.miele at bbsrc.ac.uk



More information about the Neur-sci mailing list