help in silver staining

[Sophie Duport]

hello,

i'm working on hippocampal organotypic cultures and i-m trying to stain
neurones with the golgi silver method... in vain!!!!!!
has anybody followed this method successfully?
Is there any other impregnation method available to labelled neurones?
has anybody faced the same problem as me and overcome it?

Thank you for all answers and/or advices and suggestions.





s. Duport
DPT of Pharmacology 
CMU
Geneva