Help with NGF ELISA

Robert W. Andersohn gigan at grove.ufl.edu
Wed Jan 21 20:14:32 EST 1998


I am a student researcher from the Univ. of Florida, and I have been
trying to use an "indirect sandwich" ELISA to measure NGF in postnatal
rat brain.  I have run in to some complications where our values for NGF
levels are well over the consistent values published over recent years.  
I would like to know if someone has had any problems setting up an NGF
ELISA in their lab.  I would also like to know if there are any
suggestions as to why we cannot seem to get reasonable data (ANYTHING).  
We have spent a lot of time trying to elucidate factors which could be
skewing our results and have searched the literature thoroughly.  We
have been "measuring" NGF in 4,10, and 21 day-old H-Tx rats from
striatum, hippocampus, and posterior cortex using the "NGFemax" system
from Promega Corp.  

Please respond directly by email (mailto: coriakin at grove.ufl.edu) or
post a message here. Any help would be greatly appreciated.

Jason Coleman
URL: http://grove.ufl.edu/~coriakin
 or  http://www.ufbi.ufl.edu/~joneslab





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