Help: How to reduce autofluorescence in brain sections?

Tom Fischer tmf3 at pantheon.yale.edu
Fri Jan 23 10:35:13 EST 1998


Instead of using formaldehyde, try *fresh* paraformaldehyde. The
formaldehyde off the shelf tends to autofluoresce, as does old (one day or
more) paraformaldehyde. 

On 23 Jan 1998, Ulrike Gausmann wrote:

> Since we try to perform immunofluorescence with brain
> tissue (cryosections) fixed with formaldehyde 
> autofluorescence is always a problem - doesn't matter
> which wavelength we use. I tested the treatment with
> sodium borohydride but there is no difference to the
> untreated sections.
> Does anybody know a method how to reduce the fluorescent
> background?
> 
> Any hints are welcome,
> Ulrike
> 
> -- 
> Ulrike Gausmann			| D-07743 Jena
> Institut fuer Anatomie		| Germany
> Anatomie II			| Tel +49 (0)3641 938553
> Teichgraben 7			| email ugau at mti-n.uni-jena.de
> 
> 




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