ISH Sections & ProtK Concs.
gino.miele at bbsrc.ac.uk
Sat Jun 27 05:07:06 EST 1998
Can anyone out there advise me on the size of sections I
should use to cut mouse embryos at E7.5, E8.5, E9.5 & E10.5
stages of embryonic development? What conditions would be
best to start with for the Proteinase K digestion
concentration and incubation period for these stages?
Also, the transcript I am looking for at these stages is
undetectable by Northern until E10 - I have a probe of 200bp
and a probe of 900bp - which would be best? I would prefer to
use the 900bp probe - will this be OK without hydrolysis?
Thanks in advance,
Division of Development & Reproduction,
E-mail: gino.miele at bbsrc.ac.uk
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