Need help w/autofluorescence problem

Steve Singer ssinger at orion.it.luc.edu
Thu Oct 29 18:12:49 EST 1998


Hello -

I'm doing confocal microscopy on paraffin-embedded human brain from
controls and Alzheimer's disease.  I'm getting *strong* autofluorescence
from unlabelled tissue (i.e. no antibody - no fluorochrome).  Any
suggestions?






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