i need help for brain cryostat section

arnaud2507 at my-dejanews.com arnaud2507 at my-dejanews.com
Wed Mar 3 04:32:59 EST 1999


hi,

I'm a little problem with brain cryostat section.

I want to cut mice brain with a cryostat in order to stain with thionin and
see the purkinje cells in the cerebellum (for count), but i don't know the
better fixation procedure to cut the brain at 10µm max.

For the moment, i froze the brain in isopentane. With this procedure, it's
really difficult to section it under 25µm, and at this thickness, it's
pratically impossible to counts the P.Cells, so my question is : is it
possible to fixed the brain after frozen in isopentane in order to cut it at
10µm thickness?

What is the good temperature in cryostat to cut brain tissu?

If i fixed the brain in formalin, what is the procedure, after fixation, to
cut it in cryostat?

If someone have a good procedure, please help me!!!

bye
arnaud

ps : sorry for my bad english!!!

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