I thought that MacLab is now iworx, and that BioPac is a different company.
In any case, have you tried the manufacturers web sites? (www.biopac.com or
www.iworx.com) Both of those vendors have complete lab manuals for use with
Measuring conduction velocity of the frog sciatic nerve is a fairly
traditional lab in introductory physiology or neurobiology and there are
many lab manuals and descriptions of how to do it. Basically, you just
record the action potential at two locations along the nerve and calculate
velocity as difference in distance (between the two recording sites) divided
by difference in time (latency of response at the two sites). Do not try to
measure velocity as the distance between stimulating and recording
electrodes divided by the time between stimulus and response. You don't
know exactly when or where the action potential actually started.
Frog Ringer's solution is the appropriate solution
Neuromuscular junction "assays" depend critically on exactly what type of
equipment you have available. I hesitate even to suggest how to record the
junction potential with microelectrodes if you don't even have experience
with the sciatic nerve compound action potential. The iworx animal
physiology lab manual has details, as do many physiology and neurobiology
And, of course, I assume you already have the necessary approval for working
with vertebrate animals from your campus animal care and use committee!
Brian MacNevin <macnevin at cc.wwu.edu> wrote in message
news:80q7rv$ok0$1 at ra.cc.wwu.edu...
> Hi everyone,
> I am attempting to employ BIOPAC (formerly MACLAB I believe) in
> for neurotoxicity along a frog's sciatic nerve. While we have the
> appropriate amplifier coming to us, I do not have any protocols for the
> procedure, nor for the solutions necessary for maintaining the nerve
> fascicle in the nerve box. Anyone with knowledge or experience using
> BIOPAC system, I'd appreciate pointers! URLS or citations to basic AP
> velocity assays or NMJ -> transducer assays would be GREATLY appreciated!
> Brian MacNevin
> Biology Department
> Western Washington University
> Bellingham, WA 98225-9160
>macnevin at cc.wwu.edu> Tel: (360) 650-7465