Requesting protocols on Golgi staining

Kalman Rubinson kr4 at
Thu Nov 25 11:22:09 EST 1999

i_robot at wrote:
> I would appreciate any information about where I can find DETAILED
> protocols concerning general Golgi staining techniques that can be
> performed on freely floating or slide-mounted brain sections (vibratome
> or cryostat sectioned).  I realize that many variants to the Golgi
> staining method exist, and that it is a fairly old (late 1800s - early
> 1900s) technique.  Any info, nevertheless, will be appreciated.  I have
> some papers obtained via Medline searching, but your info may be
> useful! Thank you.

I'd like to hear more about this, too.  Most Golgi impregnation
techniques are performed en bloc and result in a surface deposit 
that must be sliced/wiped away.  


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