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silverstaining

Kalman Rubinson kr4 at is2.nyu.edu
Thu Nov 25 20:23:42 EST 1999


Bohlen.Hintz at t-online.de wrote:
> To stain degenerated nerve-fibers, the Fink-Heimer-method is a good
> choice; unfortunately I have to use uranyl-nitrate for it - this
> substance is radioactive and therefore no longer  topurchase.
> Is there a substance I can use instead of uranylnitrate to archieve
> the same results?

Phosphomolybdic acid.

> > Or is there another good staining method to visualize degenerated
> > neurons?
> > There is a staining calle NAUTA - but I have not the protocol for it -
> > do you have it ?
> > And do I need uranylnitrate for it?

Both use uranyl nitrate OR PMA.

> > Do you could help me - perhaps with another staining-protocol or -
> > have you an idea how to substitute uranyl-nitrate in the
> > Fink-Heimer-protocol?

Check the older books like Nauta/Ebbesson (1971, Elsevier) or Heimer's
books.

Kal






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