in vitro nerve experiments

Stefan de Kool dekool at
Tue Apr 18 08:24:25 EST 2000

Dear anybody,

I'm doiing in vitro measurements of compound action potentials of rat
peripheral nerves. Can anyone tell me what is the best way to unsure the
nerve keeps functioning upon electrical stimulation? In literature
people use different kinds of buffer solutions and sometimes bubble
there solutions with carbonate (95%O2,5%CO2), but it's not clear to me
which way is the best. I'll be glad to hear anything.
Thanks for your attention. bye

Stefan de Kool,
Research assistant
Erasmus University Rotterdam
email: dekool at

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